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miRNA Sequence Hybridization temperature
Anti-hsa-miR-21-5p 5’DIG-lTmCmAlAmCmAlTmCmAlGmUmClTmGmAlTmA- 53 mAlGmCmUlA-DIG
Anti-hsa-miR-146a-5p 5’FAM-lAmAmClCmCmAlTmGmGlAmAmUlTmCmAlGmUm- 56 UlCmUmClA
Anti-hsa-miR-155-5p 5’DIG- lAmCmClCmCmUlAmUmClAmCmGlAmUmUlAmGm- 56 ClAmUmUmAlA-DIG
hsa-miR-21-5p UAGCUUAUCAGACUGAUGUUGA - mimic
hsa-miR-146a-5p mimic UGAGAACUGAAUUCCAUGGGUU -
hsa-miR-155-5p UUAAUGCUAAUCGUGAUAGGGGU - mimic
five
Gene Forward primer Reverse primer Amplicon size (nt)
IL-1β gcatccagctacgaatctcc gaaccagcatcttcctcagc 99
IRAK1 gcccgaggagtacatcaaga ctctgaccagccaaggtctc 93
IRAK2 cctcctctgaggcctgtgt tgatctcaatttgccacgaa 72
TRAF6 tggcattacgagaagcagtg tggacatttgtgacctgcat 95
PDCD4 tggaaagcgtaaagatagtgtgtg ttctttcagcagcatatcaatctc 91
SHIP1 gtccaaggatggttctgagg gtgacttaatgagctgcaggatt 65
ERBB4 aggagtgaaattggacacagc tctcggtatacaaactggtttcc 71
MEF2C tgatcagcaggcaaagattg tggacactgggatggagact 72
NTF3 aaaaacggttgcaggggtat ggtttgggatgttttgcact 63
PTEN gcacaagaggccctagatttc cgcctctgactgggaatagt 77
NUMB gttgtcatgggggaggtg ttgcttaagcctcaaatctgc 105
CFH cctgacctcccaatatgtaaaga ttccttaacattcccattgagg 78
EF1A atccacctttgggtcgcttt ccgcaactgtctgtctcatatcac 51
140
Supplementary Table 1 Antisense LNA and mimic pre-miRNA sequences.
l (small letter L) = LNA modification; m = 2-o-methyl modification; DIG = digoxygenin label; FAM = fluorescein.
Supplementary Table 2 Primer sequences.